Journal: Free radical biology & medicine

Article Title: Cell-based assays that predict in vivo neurotoxicity of urban ambient nanosized particulate matter

doi: 10.1016/j.freeradbiomed.2019.09.016

Figure Lengend Snippet: NF-κB activation by air pollution PMs. (A) Schema of NF-κB activation in the THP1-Blue monocyte assay; (B) NF-κB activation by 24 h (nPM and sPM, 5 μg/ml; DEP, 100 μg/ml; LPS, 10 ng/ml). (C) LPS neutralization by pretreatment with polymyxin B (10 ng/ml, 20 min) before NF-κB activation by nPM or sPM (5 μg/ml/24 h). * Adjusted p-value < 0.05, N = 4.

Article Snippet: Cell culture THP1-Blue NF-κB monocytes from InvivoGen (San Diego, CA, USA) were maintained in RPMI1640 medium containing 10% FBS, 1% pen/strep, 100 μg/ml Normocin, and 10 μg/ml Blasticidin at 4×10 5 −1×10 6 cells/ml with 5% CO 2 at 37 °C.

Techniques: Activation Assay, Neutralization

Journal: Free radical biology & medicine

Article Title: Cell-based assays that predict in vivo neurotoxicity of urban ambient nanosized particulate matter

doi: 10.1016/j.freeradbiomed.2019.09.016

Figure Lengend Snippet: nPM induced cytokines through NF-κB activation in THP1 monocytes. (A) nPM increased nuclear NF-κB p65 (Western blot) by 5 μg/ml nPM (B) Cytokine induction by nPM (RT-PCR) was suppressed by pretreatment with 40 μg/ml of SN50, a NF-κB inhibitor. *P < 0.05, **P < 0.01 vs control, #, P < 0.05, nPM alone, N = 4.

Article Snippet: Cell culture THP1-Blue NF-κB monocytes from InvivoGen (San Diego, CA, USA) were maintained in RPMI1640 medium containing 10% FBS, 1% pen/strep, 100 μg/ml Normocin, and 10 μg/ml Blasticidin at 4×10 5 −1×10 6 cells/ml with 5% CO 2 at 37 °C.

Techniques: Activation Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Free radical biology & medicine

Article Title: Cell-based assays that predict in vivo neurotoxicity of urban ambient nanosized particulate matter

doi: 10.1016/j.freeradbiomed.2019.09.016

Figure Lengend Snippet: Lipid peroxidation potency measured by DPPP assay. After incubation with DPPP (4.5 μM/15min), THP1-Blue cells were exposed to air pollution PM (nPM, sPM, DEP: 10 μg/ml), LPS (10 μg/ml), or H2O2 (200 μM) for 20 min, and fluorescence was measured (excitation, 351 nm; emission, 380 nm). * p-value < 0.05, N = 4.

Article Snippet: Cell culture THP1-Blue NF-κB monocytes from InvivoGen (San Diego, CA, USA) were maintained in RPMI1640 medium containing 10% FBS, 1% pen/strep, 100 μg/ml Normocin, and 10 μg/ml Blasticidin at 4×10 5 −1×10 6 cells/ml with 5% CO 2 at 37 °C.

Techniques: Incubation, Fluorescence

Journal: Free radical biology & medicine

Article Title: Cell-based assays that predict in vivo neurotoxicity of urban ambient nanosized particulate matter

doi: 10.1016/j.freeradbiomed.2019.09.016

Figure Lengend Snippet: In vivo neurotoxic responses of nPM paralleled in vitro NF-κB induction activity. Young male mice were exposed for 3 or 8 weeks to nPM batches with different in vitro NF-κB activity for neurotoxic responses. A) Inflammatory cytokines after 3 weeks of exposure to 300 μg/m3 of PM0.2. Heatmap shows the fold changes of inflammatory cytokines in mice exposed to particles vs controls exposed to filtered air. B) In vitro NF-κB activation potency of PM was associated with the variance of cerebral cortex inflammatory responses. Principal component analysis of the 9 inflammatory cytokines was calculated for each mouse. In vitro NF-κB response was associated with PC3 that explained 17% of the variance brain inflammatory response. Cerebral cortex inflammatory profile of mice exposed to different batches of nPM or sPM did not correlate with in vitro (C) lipid peroxidation or (D) NO production. E) nPM with stronger NF-κB activation in vitro caused more increase in cerebral cortex amyloid-β40 &-42 peptides and hippocampal Iba1. Mean ± SEM. * adjusted P-values < 0.05. N = 5–10/group.

Article Snippet: Cell culture THP1-Blue NF-κB monocytes from InvivoGen (San Diego, CA, USA) were maintained in RPMI1640 medium containing 10% FBS, 1% pen/strep, 100 μg/ml Normocin, and 10 μg/ml Blasticidin at 4×10 5 −1×10 6 cells/ml with 5% CO 2 at 37 °C.

Techniques: In Vivo, In Vitro, Activity Assay, Activation Assay

Journal: OncoTargets and therapy

Article Title: Hypermethylation of secreted frizzled-related proteins predicts poor prognosis in non-M3 acute myeloid leukemia

doi: 10.2147/OTT.S136502

Figure Lengend Snippet: Correlation between SFRP1 hypermethylation and expression level in non-M3 AML patients and HL60 cell line (since the value was lg function conversion, methylation and expression level at zero was excluded). Abbreviation: AML, acute myeloid leukemia.

Article Snippet: Seven human AML cell lines (SHI-1, THP-1, U937, HEL, HL60, K562, and NB4) (ATCC, Manassas, VA, USA) were routinely cultured in IMDM with 10% fetal bovine serum (ExCell Bio, Shanghai, People’s Republic of China) and grown at 37°C in 5% CO 2 humidified atmosphere.

Techniques: Expressing, Methylation